 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Isozymes Electrophoresis for Varietal Verification in Some Tropical Vegetables ) ผู้เขียน: Panic Temiesak, Kasemsak Palakorn, Karnda Tantiyavarong,  นายพรพันธ์ ภู่พร้อมพันธุ์ สื่อสิ่งพิมพ์:pdf AbstractTechniques for varietal identification of vegetable crops using seed protein and isozymes electrophoresis were investigated. Several isozymes namely, alcohol dehydrogenase (ADH), peroxidase, esterase, glutamic oxaloacetic transaminase (GOT), phosphoglucomutase (PGM) and malate dehydrogenase (MDH) were analysed to standardize in proper condition of plant ages, plant parts, sample extraction solution, gel concentration and staining solution. It had been found that 12 % gel was suitable to analyse isozymes and 13.5 % for protein. Isozymes extracted with phosphate buffer, pH 7.5-8.5 from leaves of 7 day old seedling showed the best expression of banding pattern and distinguish the difference among breeding lines, namely peroxidase in sweet corn, ADH, PGM and esterase in Brassica; esterase in tomato and okra |